Douglas Frey

Douglas Frey is a Collaborator, Center for Advanced Sensor Technology and a Professor, Department of Chemical and Biochemical Engineering University of Maryland, Baltimore County.



Title: Professor, UMBC

More Information: Frey Lab Website


  • Ph.D., Chemical Engineering, University of California, Berkeley, 1984.
  • M.S., Chemical Engineering, University of California, Berkeley, 1980.
  • B.S., Chemical Engineering, Stanford University, 1978.
  • B.A., Chemistry, Willamette University (combined degree program), 1978.


  • Professor, Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County (2001-present).
  • Program Director, Separation Processes Program, National Science Foundation (2001-3, 2005-6).
  • Graduate Program Director, Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (2007 – 2014).
  • Undergraduate Program Director, Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (2000-2007).
  • Department Chairman, Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County (1995-2000).
  • Associate Professor, Department Chemical and Biochemical Engineering, University of Maryland Baltimore County (1993-2001).
  • Associate Professor, Chemical Engineering, Yale University (1990-93).
  • Assistant Professor, Chemical Engineering, Yale University (1984-90).

Courses Taught

  • ENCH 427:  Heat and Mass Transfer
  • ENCH 445:  Separation Processes
  • ENCH 485L:  Biochemical Engineering Laboratory
  • ENCH 610:  Chemical Engineering Thermodynamics
  • ENCH 630:  Transport Phenomena

Research Areas

Separation methods in biotechnology and proteomics, chromatography of biological macromolecules, transport processes in porous media, dehydration processes, adsorption of macromolecules and nanoparticles onto surfaces, nanoparticle sorting, biopharmaceutical process science.

Professional Memberships and Awards

  • Editorial Board Member for Biotechnology and Applied Biochemistry (2004 – present).
  • Departmental ABET coordinator (1997-1999 and 2015-2017).
  • Consultant for W. R. Grace and Bristol-Myers Squibb.
  • Member of the American Chemical Society.
  • Member of the American Institute of Chemical Engineers.
  • Henry Ford II Scholar Award (chosen by Stanford University faculty as the outstanding graduating senior from the School of Engineering in 1978), Phi Beta Kappa.

Selected Recent Publications

More than 80 peer-reviewed articles have been published in leading journals including Biotechnology and Bioengineering, Journal of Chromatography A, Biotechnology Progress, Analytical Chemistry, AIChE Journal, Chemical Engineering Science, and Chemical Engineering Education. 

  • P. Moitra, M. Alafeef, K. Dighe, P. Ray … D.D. Frey, D. Pan, G. Rao “Rapid and Low-Cost Sampling for Detection of Airborne SARS-CoV-2 in Dehumidifier Condenste” Biotechnol. Bioeng., 118, 3029-3036-123, 2021  [abstract]
  • P. Rezaei, L.D. Pfefferle, D.D. Frey, “Metal-Semiconductor Sorting of Large-Diameter Single-Wall Carbon Nanotubes by pH-Dependent Binding to a Hydrophobic Interaction Adsorbent” Carbon, 175, 112-123, 2021  [abstract]
  • R. Adiga, A.U. Andar, S. Borhani, D. Bergenson, S. Deldari, D.D. Frey, X. Ge, Y. Liu, G. Rao, …, L. Wong, “Manufacturing Biological Medicines on Demand: Safety and Efficacy of GCSF in a Mouse Model of Total Body Irradiation” Biotechnol. Prog., 36(3), e2970, 2020  [abstract]
  • A.U. Andar, S. Deldari, E. Gutierrez, D. Bergenson, M. Al-Adhami, C. Gurramkonda, L . Telosa, Y. Kostov, D. D. Frey, G. Rao, “Low Cost Customizable Microscale Toolkit for Rapid Screening and Purification of Therapeutic Proteins” Biotechnol. Bioengin., 116, 870-881, 2019  [abstract]
  • Y. Liu, S. Deldari, H. Guo, C.R. Narahari, R.C. Bates, R. Swanson, S. Ghose, Z.J. Li, D.D. Frey “Evaluation of Chromatofocusing as a Capture Method for Monoclonal Antibody Products” J. Chromatogr. A, 1568, 108-122, 2018.  [abstract]
  • B.C. Bundy, J.P. Hunt, M.C. Jewett, J.R. Swartz, D.W. Wood, D.D. Frey, G. Rao “Cell-Free Biomanufacturing” Current Opinion Chemical Engineering, 22, 177-183, 2018.  [abstract]
  • R. Adiga, M. Al-Adhami, A. Andar, S. Borhani, S. Brown, D. Bergenson, M. A. Cooper, S. Deldari, D. D. Frey, X. Ge, H. Guo, C. Gurramkonda, P. Jensen, Y. Kostov, W. LaCourse, Y. Liu, A. Moriera, …, G. Rao, J. Wilhide, D. Wood, A. Zuber “Point-of-Care Production of Therapeutic Proteins of Good-Manufaturing-Practice Quality” Nature Biomedical Engineering, 2, 675-686, 2018.  [abstract]
  • C. Gurramkonda, A. Rao, S. Borhani, M.A. M. Pilli, S. Deldari, X. Ge, N. Pezeshk, T.-C. Han, M. Tolosa, Y. Kostov, L. Tolosa, D.W. Wood, K. Vattem, D.D. Frey, G. Rao “Improving the Recombinant Human Erythropoietin Glycosylation using Microsome Supplementation in CHO Cell-Free System” Biotechnol. Bioeng., 115, 1253-1264, 2018.  [abstract]
  • K. Tran, C. Gurramkonda, M.A. Cooper, M. Pilli, J.E. Taris, N. Selock, T.-C. Han, M. Tolosa, A. Zuber, C. Penalber-Johnson, C. Dinkins, N. Pezeshk, Y. Kostov, D.D. Frey, L. Tolosa, D.W. Wood, G. Rao “Cell-Free Expression of a Therapeutic Protein: Expression, Purification, and Characterization of Recombinant Streptokinase using a CHO Lysate” Biotechnol. Bioeng., 118, 92-102, 2018.  [abstract]
  • S. Johnson, K. A. Brorson, D. D. Frey, A K. Dhar, D. A. Cetlin “Characterization of Non-Infectious Virus-Like Particles for Viral Clearance Applications,” Applied Biochem. Biotechnol., 183, 318-331, 2017.  [abstract]
  • N. D. S. Pinto, S. D. Uplekar, A. R. Moreira, G. Rao, D. D. Frey, “Immunoglobulin G Elution in Protein A Chromatography Employing the Method of Chromatofocusing for Reducing the Co-Elution of Impurities,” Biotechnol. Bioeng., 114, 154-162, 2017.  [abstract]
  • C. Penalber-Johnson, X. Ge, K. Tran, N. Selock, N. Sardesai, C. Gurramkonda, M. Pelli, M. Tolosa, L. Tolosa, Y. Kostov, D.D. Frey, G. Rao, “”Optimizing Cell-Free Protein Expression in CHO: Assessing Small Molecule Mass Transfer Effects in Various Reactor Configurations,”” Biotechnol. Bioeng., 114, 1478-1486, 2017.  [abstract]
  • G. Rao, Y. Kostov, L. Tolosa, L. Hung, X. Ge, D. D. Frey, V. Singh, “Microscale Bioprocessing System and Method for Protein Manufacuring,” US Patent 9,388,3732, 2016.  [abstract]
  • M. M. H. El-Sayed, D. D. Frey, “Chromatofocusing” in Encyclopedia of Life Sciences, Wiley, 2015 [abstract]
  • N. D. S. Pinto, D. D. Frey, “Displacement Chromatography of Proteins Using a Retained pH Front in a Hydrophobic Charge Induction Chromatography Column,” J. Chromatogr. A, 1387, 53-59, 2015.  [abstract]
  • H. Guo, X. Li, D. D. Frey, “Development of Chromatofocusing Techniques Using Mixed-Mode Column Packings for Protein Separations,” J. Chromatogr. A, 1323, 57-65, 2014.  [abstract]
  • X. Kang, J. P. Kutzko, M. L. Hayes, D. D. Frey, “Monoclonal Antibody Heterogeneity Analysis and Deamidation Monitoring with High-Performance Cation-Exchange Chromatofocusing Using Simple, Two Component Buffer Systems,” J. Chromatogr. A, 1283, 89-97, 2013.  [abstract]
  • D. D. Frey, H. Guo, N. Karnik, “High Performance Liquid Chromatography in the Undergraduate Chemical Engineering Laboratory,” Chem. Eng. Educ. 47, 15-24, 2013.  [abstract]
  • H. Guo, D. D. Frey, “Interpreting the Difference Between Conventional and Bi-directional Plate Height Measurements in Liquid Chromatography,” J. Chromatogr. A, 1217, 6214-6229, 2010.  [abstract]
  • G. Rao, Y. Kostov, A. Moriera, D. Frey, M. Hanson, O.-W. Rief, R. Baumfalk, J. Qualitz, “Non-Invasive Sensors as Enablers of Smart Disposibles,” BioProcess International, 7, Supl. 1, 24-27, 2010. [abstract]
  • D. M. Strauss, S. Lute, Z. Tebaykina, D. D. Frey, C. Ho, S. G. Blank, K. Brorson, Q. Chen, B. Yang, “Understanding the Mechanism of Virus Removal by Q Sepharose Fast Flow Chromatography During the Purification of CHO-Cell Derived Biotherapeutics, ” Biotechnol. Bioeng. 104, 371-380, 2009.  [abstract]
  • H. Shen, D. D. Frey, “Serial Displacement Chromatofocusing and its Applications in Multidimensional Chromatography and Gel Electrophoresis: Part I. Theory and General Considerations,” J. Chromatogr. A, 1216, 967-976, 2009.  [abstract]
  • H. Shen, X. Li, C. J. Bieberich, D. D. Frey, “Serial Displacement Chromatofocusing and its Applications in Multidimensional Chromatography and Gel Electrophoresis: Part II. Experimental Results,” J. Chromatogr. A, 1216, 977-986, 2009.  [abstract]
  • K. Brorson, H. Shen, S. Lute, J. Soto Pérez, D. D. Frey, “Characterization and Purification of Bacteriophages Using Chromatofocusing,” J. Chromatogr. A, 1207, 110-121, 2008. [abstract]
  • H. Shen, X. Li, C. J. Bieberich, D. D. Frey, “Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures,” Methods. Mol. Biol., 424, 187-203, 2007. [abstract]
  • X. Ge, M. Hanson, H. Shen, Y. Kostov, K.A. Brorson, D. D. Frey, A. R. Moreria, G. Rao, ” Validation of an Optical Sensor-Based High-Throughput Bioreactor System for Mammalian Cell Culture,” J. Biotechnol., 22, 293-306, 2006. [abstract]
  • D. D. Frey, X. Kang, “New Concepts in the Chromatography of Peptides and Proteins,” Curr. Opin. Biotechnol., 16, 552-560, 2005. [abstract]
  • J. Soto Pérez, D. D. Frey, “Behavior of the Inadvertant pH Transient Formed by a Salt Gradient in the Ion-Exchange Chromatography of Proteins,” Biotechnol. Progr., 21, 902-910, 2005. [abstract]
  • H. Shen, D. D. Frey, “Effect of Charge Regulation on Steric Mass-Action Equilibrium for the Ion-Exchange Adsorption of Proteins,” J. Chromatogr. A, 1079, 92-104, 2005. [abstract]